Identification and Quantification of Rhizoctonia solani and R. oryzae Using Real-Time Polymerase Chain Reaction

Identification and quantification of Rhizoctonia solani and R. oryzae using real-time polymerase chain reaction.

Rhizoctonia solani and R. oryzae are the principal causal agents of Rhizoctonia root rot in dryland cereal production systems of the Pacific Northwest. To facilitate the identification and quantification of these pathogens in agricultural samples, we developed SYBR Green I-based real-time quantitative-polymerase chain reaction (Q-PCR) assays specific to internal transcribed spacers ITS1 and ITS...

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains

Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This stud...

Detection and quantification in real-time polymerase chain reaction

The estimation of the concentration of an infectious agent in the environment is a key step to trigger an alert when there is a biological threat. This concentration can be obtained trough a quantitative polymerase chain reaction (qPCR). Nevertheless, standard real-time procedure do not address detection delay which is a main concern in alert triggering. Therefore, we propose a method based on ...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Bayesian estimation for quantification by real-time Polymerase Chain Reaction

The aim of the Quantitative Polymerase Chain Reaction is to determine the initial amount X0 of specific nucleic acids from an observed trajectory of the amplification process, the amplification being achieved through successive replication cycles. This process depends on the efficiency {pn}n of replication of the molecules, pn being the probability that a molecule will duplicate at replication ...